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Nobel Prize CRISPR gene editing technology used


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Scientists Emmanuelle Charpentier and Jennifer Doudna have won the Nobel Prize in chemistry for their pioneering work on the gene-editing tool CRISPR. The tool has been used to engineer better crops and to try to cure human diseases. (Oct. 7)

AP Domestic

Coronavirus tests performed in labs are the gold standard for accuracy, and antigen tests are a fast and inexpensive alternative.

But backers of a third type of test, developed by a Nobel Prize winner using cutting-edge CRISPR technology, say it has the potential to be all three: rapid, accurate and inexpensive.

Although these gene-editing technology tests are still being developed and won’t be ready in the United States this year as the weather cools and demand surges, research groups recently published papers describing them as an appealing alternative as testing shortages persist amid the COVID-19 pandemic. 

Dr. Jennifer Doudna, a University of California-Berkeley researcher whose pioneering work in CRISPR earned a share of this year’s Nobel Prize in chemistry, said the test can be done quickly and doesn’t require a lab. 

“We have a ways to go before CRISPR-based diagnostics reach widespread use, but I believe we’ll see an impact during the current pandemic,” Doudna said. “Because it is simple to adjust these tests to detect other targets, the platform we’re developing now is laying the groundwork to deploy CRISPR for rapid diagnosis during future outbreaks.”

CRISPR, or clustered regularly interspaced short palindromic repeats, is a gene-editing technology studied for a wide range of uses from cancer and sickle cell disease treatments to improved food production.

In 2016, Doudna’s lab developed a way to detect RNA using the technology. Her lab collaborated with Dr. Melanie Ott of San Francisco-based Gladstone Institutes to  develop an HIV test, but when the pandemic hit, the researchers focused on developing a coronavirus test.

The test recognizes a sequence of RNA in SARS-CoV-2, the coronavirus that causes COVID-19. 

In an Oct. 12 publication, researchers reported the test yielded results in five minutes and correctly identified five samples from patients with the coronavirus. When used with a mobile phone to detect signals generated by the test, the technology could provide a fast, low-cost test outside a laboratory, researchers saidin the paper, which was not peer-reviewed. 

Massachusetts Institute of Technology scientists also are honing a CRISPR-based test that can be used outside a lab. In a New England Journal of Medicine letter published last month, researchers said the test was evaluated at a University of Washington lab using 202 samples with the coronavirus and 200 without. The test correctly identified 93.1% of positive samples. The test also had 98.5% specificity, which means it rarely reported false positives.

Feluda, a paper-based CRISPRtest named after a fictional India detective, has been cleared by that nation’s drug regulators for commercial launch. But it’s unclear how the Indian conglomerate Tata Group plans to deploy the test in India, which trails only the United States with nearly 7.7 million cases, according to data from Johns Hopkins University. 

Testing that is fast and accurate 

Gigi Gronvall, a senior scholar at the Johns Hopkins Center for Health Security, said she expects more labs will explore tests using this technology. She called it “extremely promising” because people can use the tests outside the lab.

“That has been a big challenge with testing generally,” Gronvall said. “People need to have their results pretty quickly otherwise they keep going about their day and they might be infectious and not stop having contact with other people.”

More: White House COVID-19 outbreak shows the limits of testing; even the most accurate ones can miss the coronavirus early

More: Rapid, cheap home tests: Companies attempt to make coronavirus tests widely available

More: ‘Totally unacceptable’: Testing delays force labs to prioritize COVID-19 tests for some, not others

The south San Francisco-based biotechnology firm Mammoth Biosciences, co-founded by Doudna, is working to further develop its test and make it available to labs and medical providers. The company received a National Institutes of Health grant to accelerate development.

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Mammoth CEO Trevor Martin said the company’s goal is to make a test that is fast and accurate.

“Right now you have to make this choice: Do you want something that is simple and fast or something that has the highest accuracy?” Martin said.

The most accurate lab tests are molecular-based polymerase chain reaction, or PCR, which amplify a small amount of genetic material from a nasal swab sample. Labs are limited in…



Source Website Nobel Prize CRISPR gene editing technology used

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